by Jack Norris, RD
- Calcium and Vegan Diets: Why the Fuss?
- Animal Protein and Osteoporosis
- Epidemiological Studies
- Metabolic Studies
- What Causes Osteoporosis?
- Calcium and Vitamin D
- Vitamin B12 and Bone Mineral Density
- Research on Vegans
- Calcium Absorption from Plant Foods
- Calcium Tips
- Dangers of Calcium Supplements
Calcium and Vegan Diets: Why the Fuss?
Americans are regularly being urged to consume more calcium in order to prevent osteoporosis. It is practically impossible to meet the recommendations without large amounts of cows’ milk, calcium-fortified foods, or supplements.
Enter vegan diets. Because vegans do not eat dairy products, their calcium intakes tend to be low. The Dietitian’s Guide to Vegetarian Diets (2004) lists 45 studies that have surveyed vegetarians’ calcium intakes in Appendix G. The daily calcium intakes in these studies are about:
- Vegans: 500 – 600 mg
- Lacto-ovo vegetarians: 800 – 900 mg
- Non-vegetarians: 1,000 mg
Only one study included supplements. In it, daily calcium intakes were: 840 mg (vegan males), 720 mg (non-vegetarian males), 710 mg (vegan females), and 855 mg (non-vegetarian females).
Animal Protein and Osteoporosis
As you can see above, the calcium intake of vegans tends to be quite a bit lower than lacto-ovo vegetarians, non-vegetarians, and the DRI. Traditionally, the vegan community has responded to this by saying osteoporosis is a disease of calcium loss from the bones, not a lack of calcium in the diet. This was based on two bits of evidence. The first bit is that ecological studies have shown that the countries with the highest intake of dairy products (northern Europe and the USA) have higher rates of hip fractures than do Asian and African countries where much less milk is consumed. This can be explained by the second bit of evidence which is that studies show that after ingesting animal protein, people urinate large amounts of calcium. Therefore, the thinking goes, calcium intake isn’t important for preventing osteoporosis and vegans are protected due to the lack of animal protein in their diets.
Let’s examine this evidence.
While it is true that Asian countries have lower rates of hip fractures than do higher dairy-consuming countries, it turns out that hip fracture rates can be misleading. The first prospective cohort study measuring clinically diagnosed vertebral fractures in an Asian population, the Hong Kong Osteoporosis Study, found that while hip fracture rates were lower in Hong Kong than Sweden, vertebral fracture rates were higher (Bow 2012), as shown in the image below.
The authors of the Hong Kong Osteoporosis Study state:
The observed ethnic differences in fracture incidences may be due to the fact that hip fracture risk was affected by fall risk, whereas the risk of vertebral fracture mostly depends on bone strength. Despite the low hip fracture rate in our population, Hong Kong women had a higher prevalence of osteoporosis [technical parenthetical deleted] than US Caucasian women (35.8% vs. 20%, respectively) and a similar prevalence of about 6% in Hong Kong and US Caucasian men.
In other words, at least in this group of people from Hong Kong, the Chinese don’t have lower rates of osteoporosis.
Here are some excerpts from a 2009 review of the epidemiological studies on protein and bone health (Darling 2009):
Overall, there was very little evidence of a deleterious influence of protein intake on [bone mineral density (BMD)], with most cross-sectional surveys and cohort studies reporting either no influence or a positive influence. Thus, 15 cross-sectional surveys found a statistically significant positive relation between protein intake and at least one BMD site. However, 18 studies found no significant correlation between protein intake and at least one BMD site.
The cohort studies also identified little evidence of any deleterious influence of protein intake on bone. …[N]o studies showed a significant increase in BMD loss with increased protein intake, and only one study showed a significant decrease in BMD loss with increased animal and total protein intakes.”
Overall, the [seven] cohort studies indicated either a benefit or no effect of protein intake on hip fracture relative risk, with only one study reporting a significant increase in risk with increasing animal protein intake and increasing animal to vegetable protein ratio. Three studies found a decreased relative risk of hip fracture with increasing animal, total, and vegetable protein intakes. Two studies found no significant association of animal protein with fracture risk, whereas 2 studies found no association of total protein with fracture risk. Last, 2 studies found no relation between fracture risk and vegetable protein.
In a large observational study (2014), researchers in Sweden found milk consumption to be positively associated with early death in both men and women, as well as fractures—especially hip fractures—in women. While statistically significant, differences weren’t large enough to indicate dairy plays more than a small role in early death or fractures. In analyses of three sub-cohorts, milk consumption was also found to be associated with oxidative stress in men and women as well as inflammation in men (Michaëlsson 2014).
As mentioned above, there is a theory that protein increases osteoporosis by increasing a loss of calcium through the urine. The idea is that protein, especially through the sulfur-containing amino acids, increases the acid in the blood which, in turn, gets shuttled to the urine, increasing the renal acid load. In order to neutralize that acid, calcium is used as a buffer and then urinated out with the acid.
There has been an enormous amount of research on protein and bone health and this view of protein causing osteoporosis has been refuted. Here are some of the main points of a 2012 literature review (Calvez 2012) of clinical trials on the subject:
- Many clinical trials show that adding purified proteins to the diet increases calcium excretion through the urine. But when whole foods are eaten, this effect is not strong. And phosphorus, in which meat and dairy are rich, counteracts the increase of calcium in the urine between 40 and 65%.
- High protein diets increase acid excretion in the urine, but this can be handled by the body’s acid buffer system without the need for calcium.
- In low-calcium, but not high-calcium diets, higher protein intakes probably increase calcium absorption from the digestive tract causing an increase in calcium excretion in the urine.
- Fruits and vegetables are beneficial to bone health, probably due to their high potassium and magnesium content. This could cause confounding in protein studies because diets high in protein are often low in fruits and vegetables.
- There is some evidence that a beneficial effect of protein on bones is only seen when calcium intake and vitamin D status is adequate.
- Maintenance of adequate bone strength and density with aging is dependent on adequate muscle mass which is dependent on adequate intake of protein.
- An increase in IGF-1 is most likely the mechanism for increased bone health with higher protein intakes.
Although HP [high protein] diets induce an increase in net acid and urinary calcium excretion, they do not seem to be linked to impaired calcium balance and no clinical data support the hypothesis of a detrimental effect of HP diet on bone health, except in the context of inadequate calcium supply.
Another 2009 meta-analysis found that among five well-designed studies measuring calcium balance, net acid excretion was not associated with either decreased calcium balance or a marker of bone deterioration (Fenton 2009).
What Causes Osteoporosis?
If animal protein doesn’t cause osteoporosis, what does?
Genetics likely play a strong role in osteoporosis, and, possibly related, estrogen levels in women. Among factors that can be controlled by lifestyle and diet, there has been evidence for the following:
- Weight-bearing exercise throughout one’s lifetime
- Higher body weight
- Adequate intake of calcium, vitamin D, vitamin K, phosphorus, potassium, magnesium, and boron
- High sodium and caffeine intake
- Too little protein
- Excessive vitamin A (retinol, not beta-carotene)
- Possibly vitamin B12 deficiency
Calcium and Vitamin D
Some background: When calcium levels in the blood drop, parathyroid hormone (PTH) is released. PTH causes calcium to be released from the bones, thus raising the low calcium levels in the blood. Osteoporosis may result from chronically high levels of PTH. The conversion of 25-hydroxyvitamin D to calcitriol is also somewhat regulated by PTH levels (Groff 2000). Calcitriol increases absorption of calcium and phosphorus (another major component of bones) from the intestines and decreases their excretion in the urine. In so doing, calcium levels in the blood rise and PTH levels drop.
Research has shown that, on average, Americans are getting enough calcium. A 2003 report from the Nurses Health Study showed vitamin D to be more important than calcium intake for preventing hip fractures in postmenopausal women (Feskanich 2003). In 2007, a meta-analysis of prospective studies and randomized controlled trials found that calcium intake and calcium supplements were not associated with a lower risk for hip fractures (Bischoff-Ferrari 2007).
Vitamin B12 and Bone Mineral Density
Taking vitamin B12 might also be important for bone mineral density. Two studies have linked low B12 status in vegetarians to poorer bone health.
A 2009 cross-sectional study from Slovakia compared lacto-ovo vegetarian women to omnivores. They found that the vegetarians’ higher homocysteine levels (16.5 vs. 12.5 µmol/l; 78% vs. 45% were elevated) and lower vitamin B12 levels (246 vs. 302 pmol/l; 47% vs. 28% were deficient) were associated with significantly lower bone mineral density in the femur (Krivosikova 2009). Participants were not allowed to have been taking vitamin or mineral supplements. The researchers did not measure calcium intake or vitamin D status.
Another 2009 cross-sectional study of German omnivores, lacto-ovo vegetarians, and omnivores, and Indian lacto-ovo vegetarians and omnivores found higher markers of bone turnover associated with low vitamin B12 status (Herrmann 2009). The findings were no worse for the lacto-ovo vegetarians than the vegans, indicating that poor bone health can start with just moderate B12 deficiency. None of the participants were taking B12, calcium, or vitamin D supplements.
Research on Vegans
Bone Mineral Density
A number of small, cross-sectional studies have shown vegans to have the same or slightly lower bone mineral density as non-vegans (Barr 1998, Lau 1998, Parsons 1997, Ho-Pham 2009a). These studies were done on vegans who might not have gotten much vitamin D and probably did not make an effort to get the recommended amount of calcium in their diet.
In 2009, researchers from Vietnam and Australia did a meta-analysis looking at the bone mineral density of vegetarians (Ho-Pham 2009b). They concluded that “[T]here is a modest effect of vegetarian diets, particularly a vegan diet, on [bone mineral density], but the effect size is unlikely to result in a clinically important increase in fracture risk.”
A Denmark (2018) study found that vegans had higher levels of some, but not all, markers of bone turnover, even though they had relatively similar calcium and vitamin D intakes to omnivores (Hansen 2018). It’s not clear if these markers were of clinical significance.
In 2007, the first study looking at vegan bone fracture rates was released (Appleby 2007). The EPIC-Oxford study recruited 57,000 participants, including over 1,000 vegans and almost 10,000 lacto-ovo vegetarians, from 1993 to 2000. They were asked to fill out a questionnaire to measure what they ate. About 5 years after entering the study, they were sent a follow-up questionnaire asking if they had suffered any bone fractures.
After adjusting for age alone, the vegans had a 37% higher fracture rate than meat-eaters (RR 1.37, CI 1.07–1.74). After adjusting for age, smoking, alcohol consumption, body mass, physical activity, marital status, births, and hormone replacement, vegans had a 30% higher fracture rate (RR 1.30, CI 1.02-1.66). Meat-eaters, fish-eaters, and lacto-ovo fracture rates did not differ in any of the analyses performed.
When the results were adjusted for calcium intake, the vegans no longer had a significantly higher fracture rate (RR 1.15, CI .89-1.49). And among the group of subjects who got at least 525 mg of calcium a day (only 55% of the vegans compared to about 95% of the other diet groups), vegans had the same fracture rates as the other diet groups (RR 1.00, CI .69-1.44). The study didn’t give the average calcium intake of those vegans, but it was possible to calculate that at a minimum, their average calcium intake was 640 mg.
The authors noted that fracture rates did not correlate with protein or vitamin D intake among the people in this study. A separate analysis in EPIC-Oxford (Key 2007) showed that, among all participants (regardless of diet group), calcium intake was related to an increased fracture risk in women (relative risk 1.75 (1.33-2.29) for < 525 mg/day compared to > 1200 mg/day), but not in men.
Adventist Health Study-2 (2013)
Adventist Health Study-2 (AHS-2) published a report of caucasian Seventh-day Adventists living in the USA, comparing the intakes of many different foods and hip fracture risk after 5 years of follow-up (Lousuebsakul-Matthews 2013). The point of the study was not to determine the difference in fracture rates between vegetarians and non-vegetarians and they did not test for statistical significance. However, there was a strong trend towards higher fracture rates in vegans (3.0 per 1,000 person-years) compared to 2.0 for non-vegan vegetarians (including semi-vegetarians) and 1.6 for non-vegetarians.
The study found that eating meat alternatives once a day or more (compared to less than once per week) was associated with a 66% reduced risk of hip fracture in the vegetarians (.34, .12-.95). Even more strongly, eating legumes once a day or more (compared to less than once per week) was associated with a 55% reduced risk in vegetarians (.45, .22-.94).
The authors state, “Protein is recognized for its ability to improve [calcium] balance, suppress parathyroid hormone, increase lean body mass and increase production of the bone growth regulator insulin-like growth factor-1.”
Buddhist Nuns (Vietnam, 2011)
In 2011, a follow-up (Ho-Pham 2011) of an earlier study on vegan Buddhist nuns (Ho-Pham 2009a) was released. After two years, the vertebrae of 88 vegans and 93 omnivores were examined using x-rays. Ten women (five vegans and five omnivores) had sustained a new vertebral fracture after two years; there was no significant difference between the two groups.
Rates of bone mineral density (BMD) change were examined at the lumbar spine and femoral neck with a variety of associations found. Lumbar BMD increased with age, lean body mass, and vegetable fat; and decreased with vegetable protein and steroid use. The authors suggested that the increase in BMD of the lumbar spine was possibly due to osteoarthritis and, therefore, not a healthy phenomenon.
As for the femoral neck, BMD increased with both lean and fat body mass; and decreased with age, animal fat, and ratio of animal protein to vegetable protein. This would indicate that animal protein had a negative impact on bone. To make this even a bit more complicated, the food questionnaires used by the researchers indicated that the vegans were only eating an average of 1,093 calories, 36 g of protein, and 360 mg of calcium per day. The estimated energy requirement for women their age and size is about 1,600 calories which indicates that the food intake of the vegans was possibly underestimated by one-third. The non-vegan nuns had intakes of 1,429 calories, 62 g of protein, and 590 mg of calcium per day which seems more likely.
Ten fractures in 181 women in two years seemed high. The rate of vertebral fracture in women over 65 in Hong Kong and Japan is 594/100,000 person-years (Ho-Pham 2009a). The person-year fracture rate in this Buddhist nun study works out to be 2,762/100,000. That’s obviously quite a bit higher, but it should be noted that the fractures in the Buddhist nun study were determined by giving an x-ray to each subject, rather than reporting a bone break, and would likely find more fractures than the study determining the 594/100,000 rate.
In summary, compared to non-vegetarian Buddhist nuns, vegan nuns had a similar rate of vertebral fractures, but it appears that the rates for both groups were relatively high.
Some research has linked calcium supplements with an increased risk of cardiovascular disease. These studies have mostly found a link in people getting well over the DRI for calcium (more info), and with the effect stronger in smokers. A 2013 study from Sweden found that there was no increased risk up to 1,300 mg of calcium per day, but a significantly increased risk for the group getting ≥ 1,400 mg per day (Michaëlsson 2013). A 2012 study that examined the arteries of participants found no correlation with calcium supplements and calcification of the arteries in amounts up to 3,000 mg per day (Samelson 2012). To be safe, it might be best not to increase calcium intake higher than 1,400 mg per day. People with chronic kidney disease should talk to their physicians about the costs and benefits of calcium supplements.
There has been some concern that taking calcium supplements can increase the risk of kidney stones, but for the most part research has shown that taking calcium supplements with meals can actually reduce the risk of calcium-oxalate kidney stones, the most common form of stone. For more information on this, see the article Oxalate.
Calcium Absorption from Plant Foods
For calcium amounts and absorption rates of various plant foods, please see the table Calcium and Oxalate Content of Foods. Most of the calcium amounts in that table come from the USDA nutrient database, which lists an average of the samples they’ve analyzed. The amount of calcium in plant foods is somewhat dependent on the amount of calcium in the soil (Weaver 2013).
The DRI for calcium is roughly based on an average absorption of calcium from foods of 25% (p. 38 in reference DRI Dietary Reference Intakes 2011). This means adults should aim to absorb about 250 mg of calcium per day.
As a calcium dose increases, the percentage absorbed decreases. Beyond the size of the dose, the oxalate level in a plant food is the main determinant of how much calcium can be absorbed.
See Calcium and Oxalate Content of Foods for how much calcium is absorbed from various plant foods, along with references and details for this summary of calcium in plant foods:
- Studies have shown that calcium in fortified soymilk, bok choy, kale, and mustard greens is absorbed well.
- Based on oxalate levels, the calcium in turnip greens, watercress, and broccoli should also be absorbed well.
- Based on oxalate levels, the calcium in collards should be absorbed moderately well.
- Studies have shown that the calcium in spinach and rhubarb is not absorbed well.
- Based on oxalate levels, the calcium in beet greens and swiss chard should not be absorbed well.
- Many non-dairy milks are now fortified with calcium, vitamin D, and/or vitamin B12.
Many orange juices are fortified with calcium.
- Fortified beverages should be well-shaken to make sure the calcium has not settled to the bottom of the carton.
- Calcium supplements can inhibit iron absorption if eaten at the same time.
- In addition to the calcium in the leafy greens listed on the right, leafy greens also contain
vitamin K, which is good for bones.
- The Daily Value for calcium on food labels is 1,000 mg. Therefore, if a
food label says it has 25% of the daily value, it means it has 250 mg of calcium per
Dangers of Calcium Supplements
Researchers in the Framingham Study measured calcium intakes and then followed participants for four years at which time they measured the amount of calcification of their arteries (Michaëlsson 2013). They found no correlation or trends with calcium intake and calcification of the arteries in amounts up to about 3,000 mg per day in either men or women. Ditto for calcium supplements of 500 mg per day or more compared to 0 or 1-500 mg per day.
The authors of the study note one other study looking at calcification of the arteries which found no association in the prospective arm, though did find a cross-sectional correlation at baseline (Wang 2010).
The article No Need to Worry About Calcium and Your Heart from the Tufts Health & Nutrition Letter (March 2013) makes it sound like the jury is in and it’s a done deal – calcium supplements are safe (in the amounts studied). Based on the other research I’ve written about, Calcium Supplements and Cardiovascular Disease in the News, I would still suggest not going over 1,400 mg per day.
An additional concern is with colorectal cancer. As described in Calcium Supplements and Colorectal Cancer, one study found that long-term use of a 1,200 mg per day calcium supplement was associated with an increased risk of one type of polyp that can lead to colorectal cancer. This increased risk with higher calcium intakes was not seen in other studies although the other studies didn’t looked at total calcium intake rather than supplements specifically. The authors of this study suggest that those with serrated polyps of the colon or rectum, especially women and smokers, avoid calcium supplements (Crockett 2018).
Conclusion on Calcium and Vegan Diets
There is no reason to think that vegans are protected from osteoporosis more than other diet groups, and they should strive to meet calcium recommendations. Although it is possible to meet the calcium recommendations by eating greens alone (see chart), the average vegan probably will not meet recommendations without drinking a glass of fortified drink each day, eating calcium-set tofu, or taking a 250 – 300 mg supplement (in addition to eating an otherwise balanced diet). Although it is important to get enough calcium, do not get more than 1,400 mg of calcium per day.
Last updated: June 2018
Barr 1998. Barr SI, Prior JC, Janelle KC, Lentle BC. Spinal bone mineral density in premenopausal vegetarian and nonvegetarian women: cross-sectional and prospective comparisons. J Am Diet Assoc 1998 Jul;98(7):760-5.
Bischoff-Ferrari 2007. Bischoff-Ferrari HA, Dawson-Hughes B, Baron JA, Burckhardt P, Li R, Spiegelman D, Specker B, Orav JE, Wong JB, Staehelin HB, O’Reilly E, Kiel DP, and Willett WC. Calcium intake and hip fracture risk in men and women: a meta-analysis of prospective cohort studies and randomized controlled trials. Am J of Clin Nutr. 2007 Dec;86( 6): 1780-1790.
Bow 2012. Bow CH, Cheung E, Cheung CL, Xiao SM, Loong C, Soong C, Tan KC, Luckey MM, Cauley JA, Fujiwara S, Kung AW. Ethnic difference of clinical vertebral fracture risk. Osteoporos Int. 2012 Mar;23(3):879-85.
Crockett 2018. Crockett SD, Barry EL, Mott LA, et al. Calcium and vitamin D supplementation and increased risk of serrated polyps: results from a randomised clinical trial. Gut. 2018 Mar 1. [Epub ahead of print]
Darling 2009. Darling AL, Millward DJ, Torgerson DJ, Hewitt CE, Lanham-New SA. Dietary protein and bone health: a systematic review and meta-analysis. Am J Clin Nutr. 2009 Dec;90(6):1674-92. Epub 2009 Nov 4.
DRI Dietary Reference Intakes 2011. DRI Dietary Reference Intakes Calcium Vitamin D. Committee to Review Dietary Reference Intakes for Vitamin D and Calcium. Food and Nutrition Board. A. Catharine Ross, Christine L. Taylor, Ann L. Yaktine, and Heather B. Del Valle, Editors. Institute Of Medicine of The National Academies. The National Academies Press. Washington, D.C. 2011.
Hansen 2018. Hansen TH, Madsen MTB, Jørgensen NR, Cohen AS, Hansen T, Vestergaard H, Pedersen O, Allin KH. Bone turnover, calcium homeostasis, and vitamin D status in Danish vegans. Eur J Clin Nutr. 2018 Jan 23.
Herrmann 2009. Herrmann W, Obeid R, Schorr H, Hübner U, Geisel J, Sand-Hill M, Ali N, Herrmann M. Enhanced bone metabolism in vegetarians–the role of vitamin B12 deficiency. Clin Chem Lab Med. 2009;47(11):1381-7.
Ho-Pham 2009a. Ho-Pham LT, Nguyen PL, Le TT, Doan TA, Tran NT, Le TA, Nguyen TV. Veganism, bone mineral density, and body composition: a study in Buddhist nuns. Osteoporos Int. 2009 Apr 7. (Epub ahead of print)
Ho-Pham 2011. Ho-Pham LT, Vu BQ, Lai TQ, Nguyen ND, Nguyen TV. Vegetarianism, bone loss, fracture and vitamin D: a longitudinal study in Asian vegans and non-vegans. Eur J Clin Nutr. 2011 Aug 3. (Epub ahead of print)
Iguacel I, Miguel-Berges ML, Gómez-Bruton A, Moreno LA, Julián C.Veganism, vegetarianism, bone mineral density, and fracture risk: a systematic review and meta-analysis.Nutr Rev. 2019 Jan 1;77(1):1-18. Not cited.
Key 2007. Key TJ, Appleby PN, Spencer EA, Roddam AW, Neale RE, Allen NE. Calcium, diet and fracture risk: a prospective study of 1898 incident fractures among 34 696 British women and men. Public Health Nutr. 2007 Nov;10(11):1314-20.
Krivosikova 2009. Krivosikova Z, Krajcovicova-Kudlackova M, Spustova V, Stefikova K, Valachovicova M, Blazicek P, Nemcova T. The association between high plasma homocysteine levels and lower bone mineral density in Slovak women: the impact of vegetarian diet. Eur J Nutr. 2009 Oct 7.
Lousuebsakul-Matthews 2013. Lousuebsakul-Matthews V, Thorpe DL, Knutsen R, Beeson WL, Fraser GE, Knutsen SF. Legumes and meat analogues consumption are associated with hip fracture risk independently of meat intake among Caucasian men and women: the Adventist Health Study-2. Public Health Nutr. 2013 Oct 8:1-11. (Epub ahead of print)
Michaëlsson 2013. Michaëlsson K, Melhus H, Warensjö Lemming E, Wolk A, Byberg L. Long term calcium intake and rates of all cause and cardiovascular mortality: community based prospective longitudinal cohort study. BMJ. 2013 Feb 12;346:f228.
Parsons 1997. Parsons TJ, van Dusseldorp M, van der Vliet M, van de Werken K, Schaafsma G, van Staveren WA. Reduced bone mass in Dutch adolescents fed a macrobiotic diet in early life. J Bone Miner Res 1997 Sep;12(9):1486-94.
Samelson 2012. Samelson EJ, Booth SL, Fox CS, Tucker KL, Wang TJ, Hoffmann U, Cupples LA, O’Donnell CJ, Kiel DP. Calcium intake is not associated with increased coronary artery calcification: the Framingham Study. Am J Clin Nutr. 2012 Dec;96(6):1274-80.
Wang 2010. Wang TK, Bolland MJ, van Pelt NC, Horne AM, Mason BH, Ames RW, Grey AB, Ruygrok PN, Gamble GD, Reid IR. Relationships between vascular calcification, calcium metabolism, bone density, and fractures. J Bone Miner Res. 2010 Dec;25(12):2777-85. doi: 10.1002/jbmr.183. Epub 2010 Jul 16. Erratum in: J Bone Miner Res. 2011 Feb;26(2):439. (Abstract)